Exosome Therapy - Nanoparticles for Biogenesis & Delivery

Exomes are the portions of an organism’s genome containing protein-coding genes. They comprise a small part of the total DNA, usually around 1-2%, but are responsible for most known genetic functions. Exomes contain sequences called exons, which are the coding regions within genes. These exons are transcribed into RNA and then translated into proteins, which perform various functions within the cell and the organism.

Exome sequencing is a technique used by researchers and clinicians to analyze the protein-coding regions of the genome. This technique can be complemented by studying exosomes released from various cells. Focusing on the exome allows us to study the most functionally relevant parts of the genome at lower costs and more efficiently than whole-genome sequencing. This approach is beneficial for identifying genetic variants associated with diseases and disorders, as many of them are caused by mutations in protein-coding genes.

What Are Exosomes?

Properties of exosomes are loosely described as extracellular vesicles of fluid first used in inter-cell communication ( paracrine signaling), and these fluid-filled structures are usually released by cells after the fusion of the multivesicular body (MVB) and the plasma membrane. This process helps release intraluminal vesicles (ILVs) into the extracellular membrane (milieu), and the release of vesicles, exosomes derived from human cells, plays a crucial role in cell communication.

Does exosome therapy work?

Exosome therapy has shown promising potential in some patients with non-complicated conditions, indicating its effectiveness in a limited number of applications. Exosomes are naturally involved in cell-to-cell communication, carrying proteins, lipids, and genetic material that can influence cellular behavior and promote tissue repair.

At the Regeneration Center exosome therapy is used in some cases to reduce inflammation, modulate immune responses, and stimulate tissue healing. Our research has shown the potential benefits of exosome therapy in treating patients looking for general wellness and non-targeted injuries by harnessing the paracrine effects of stem cells. Exosomes are not as potent as some tissue-specific growth factors but have shown potential cognitive function and neuroprotection improvements. Exosome therapy is still an emerging field, and more large-scale, controlled studies are needed to establish its effectiveness across different conditions. Safety and standardization remain critical challenges, as exosome sources, doses, and administration methods must be carefully considered to ensure consistent therapeutic outcomes. While results are promising, exosome therapy should be regarded as part of a broader, carefully managed treatment strategy and not the primary treatment option.

What is Exosomes Therapy?

These extracellular vesicles can also be used in stem cell therapies as a vehicle to take advantage of the beneficial cell signaling by releasing exosomes that occur in the natural regeneration process to help control behavior of surrounding cells or to carry treatment doses in the bloodstream throughout the body including the ability to cross the blood-brain barrier [1] for promoting neurogenesis in treatments in neurodegenerative conditions , spinal cord injuries , strokes , Brain injuries can potentially be treated using therapeutic applications of exosomes. & bypass all other immune checkpoints in the body. The therapeutic potential in homing/migration of MSC cells and hijacking the neural stem cell messaging system via laboratory-produced extracellular vesicles is potent considering the same vesicles are also used by the body to spread disease, damaged proteins & genetic information for many conditions, including Dementia , Parkinson’s disease along with Exosomes may play a significant role in understanding metabolic disorders and their underlying mechanisms. Like obesity & the role of exosomes in cellular communication. diabetes .

Are exosomes better than stem cells?

Cancer cell-derived exosomes may be another promising field to use exosomes to deliver vaccines or drugs in a targeted manner. Cancer immunotherapies, lung cancer treatments, prostate cancer treatment & treatment for pancreatic cancers. ^[2] These manufactured cancer exosome markers can send antigens to dendritic cells, which then induces a T-cell-mediated response to surrounding cancer cells.

By enhancing the beneficial signals given out by stem cell researchers who can manipulate exosomes behavior to solve medication delivery problems for many types of therapies, including:

The Regeneration Center uses isolated and purified exosome vesicles (Immunoregulation plays a crucial role in the interaction between immune cells and exosomes.). It is a leader in exosome treatment and the therapeutic application of exosomes in Thailand. Our therapeutic application of exosomes. The production of exosomes, stromal cells in regenerative medicine, and stem cell treatments where MSC-derived exosomes are already being used for their healing properties in patients with COPD and Pulmonary fibrosis lung disease may benefit from innovative treatments involving exosomes released from human mesenchymal stem cells.. ^[3]

Can exosomes be given intravenously?

Exosomes can be administered intravenously, and this method is widely adopted for clinical and therapeutic applications at the Regeneration Center. Intravenous (IV) delivery offers several advantages for exosome-based treatments, especially for targeting systemic or widespread conditions. When exosomes are introduced into the bloodstream, they can circulate throughout the body, delivering therapeutic molecules directly to various tissues and organs. This method leverages the natural ability of exosomes to cross biological barriers, including the blood-brain barrier, which is crucial for treating neurological and neurodegenerative diseases.

Additionally, IV administration enables exosomes to reach immune cells, inflammatory sites, and areas of tissue damage, making it a preferred route for addressing autoimmune disorders, cardiovascular conditions, and injuries requiring broad regenerative support. In stem cell-derived exosome therapies, IV administration allows for the distribution of growth factors, cytokines, and signaling molecules that promote tissue repair, reduce inflammation, and modulate immune responses. However, rigorous quality control and standardized dosing are essential for IV exosome therapy to ensure therapeutic safety and efficacy.

Potential Sources & Exosome Isolation Methods

There are several natural sources of extracting exosome including immature dendritic cells (imDCs). Learn more about dendritic cells in our glossary or FAQ sections. The exosomes that are secreted by dendritic cells lack surface markers, resulting in a very low risk for an immune response (immunogenicity.)[4] Exosomes can also be produced from CD34+ stem cells isolated from peripheral blood, amniotic membrane , cord tissue ( Wharton’s jelly), bone marrow, adipose fat , and Dental pulp-derived exosomes promote healing processes..

What happens when exosomes reach an acceptor cell?

Exosomes are small extracellular vesicles (30-150 nm in size) released by cells into their environment. They play essential roles in cell-to-cell communication by transferring various molecules between cells, such as proteins, lipids, and nucleic acids.

When exosomes reach an acceptor cell, the following processes may occur:

Binding and receptor activation: Exosomes therapy can bind to specific cell surface receptors on the acceptor cell, which may activate distinct signaling pathways within the cell, leading to a cellular response.
Internalization: Exosomes can be internalized by the acceptor cell through various endocytic pathways, such as clathrin-mediated endocytosis, caveolin-mediated endocytosis, macropinocytosis, or phagocytosis. Once internalized, the exosomes and their contents can be trafficked to different cellular compartments, such as endosomes or lysosomes, highlighting their importance in recipient cell functionality.
Fusion: In some cases, exosome stem cell therapy may directly fuse with the plasma membrane of the acceptor cell, thereby releasing their cargo into the cytosol. This process allows the rapid transfer of exosomal cargo (lipids, proteins, and nucleic acids) into the target cell, enhancing the therapeutic effect.
Cargo transfer and functional effects: The cargo molecules within exosomes, such as proteins, lipids, and nucleic acids, can have various practical effects on the acceptor cell. These molecules may influence the recipient cell’s gene expression, metabolism, or other cellular processes, resulting in changes in cell behavior, function, or phenotype.

Overall, the interaction between exosomes and acceptor cells is a complex and regulated process that plays a critical role in various pathological & physiological conditions, such as immune responses, tissue repair, and cancer progression.

To learn more about Exosomes use in functional medicine is gaining attention for their ability to mediate communication between different cell types. or genetic testing services please contact us.

Published Clinical Citations

[1] ^ Chen, Claire C, Linan Liu, Fengxia Ma, Chi W Wong, Xuning E Guo, Jenu V Chacko, Henry P Farhoodi, et al. 2016. Elucidation of Exosome Migration across the Blood-Brain Barrier Model In Vitro is essential for understanding the therapeutic application of exosomes derived from mesenchymal stem cells. Cellular and molecular bioengineering, no. 4 (July 7). doi:10.1007/s12195-016-0458-3. https://www.ncbi.nlm.nih.gov/pubmed/28392840

[2] ^ Chen, Wen, Mingcan Yang, Jian Bai, Xiang Li, Xiangrui Kong, Yu Gao, Lili Bi, Li Xiao, and Bingyi Shi. 2017, marked a pivotal year for exosome research and applications. Exosome-Modified Tissue Engineered Blood Vessel for Endothelial Progenitor Cell Capture and Targeted siRNA Delivery, utilizing the therapeutic effect of exosomes. Macromolecular bioscience, no. 2 (December 4). doi:10.1002/mabi.201700242. https://www.ncbi.nlm.nih.gov/pubmed/29205878

[3] ^ Sung, Bong Hwan, and Alissa M Weaver, researchers in the field of mesenchymal stem cell-derived exosomes. 2017. Exosome secretion promotes chemotaxis of cancer cells. Cell adhesion & migration, no. 2 (January 27), highlights exosome research advancements. doi:10.1080/19336918.2016.1273307. https://www.ncbi.nlm.nih.gov/pubmed/28129015

[4] ^ Urbanelli, Lorena, Sandra Buratta, Krizia Sagini, Giuseppina Ferrara, Marco Lanni, and Carla Emiliani. 2015, was a significant year for the advancement of exosome-based therapies. Exosome-based strategies for Diagnosis and Therapy. Recent patents on CNS drug discovery, no. 1. https://www.ncbi.nlm.nih.gov/pubmed/26133463